People from six mainly Muslim countries and all refugees now face tougher US entry due to President Donald Trump's controversial travel ban.
It means people without "close" family or business relationships in the US could be denied visas and barred entry.
Lawyers took up positions at US airports, offering free advice.
But there was no sign of the chaos that affected travellers when the first version of the ban was brought in at a few hours' notice in January.
That is because the executive order does not affect people who already have valid visas or green cards.
The Department of Homeland Security said it expected "business as usual at our ports of entry".
Monday's Supreme Court ruling upheld the temporary ban, a key Trump policy.
But the judges provided a major exception, for those who have "a credible claim of a bona fide relationship" with someone in the US.
The effect is that citizens of the affected countries with a close relative in the US, such as a spouse, parent, child or sibling, will potentially be allowed in.
In a last minute change, the Trump administration extended the definition of close family to include fiancés.
However grandparents, aunts, uncles, nephews and nieces are not considered to be "bona fide" relations.
The rules apply to people from Iran, Libya, Syria, Somalia, Sudan and Yemen, as well as to all refugees.
Moments before the ban began at 20:00 Washington time on Thursday (00:00 GMT), it emerged that the state of Hawaii had asked a federal judge for clarification.
Hawaii's attorney general has argued that the definition of "close family" is too narrow and may improperly prevent people from travelling to the US.
The American Civil Liberties Union (ACLU) said it would be closely monitoring the guidance and implementation of the order.
The Supreme Court is expected to make a final decision on the ban when its next session begins in October.
According to the new rules, for the next 90 days those from the six countries without a close relationship will not be able to enter the US.
IN - a parent, spouse, fiancé, child, son- or daughter-in-law, or sibling, including step- or half-siblings.
OUT - grandparents, aunts, uncles, nieces, nephews, in-laws, extended family and grandchildren.
Also exempt from the new rules are those with business or educational ties to the US.
However, the guidelines specifically state that the relationship must be formal, documented and not formed for the purpose of evading the order.
As well as making clear that those with valid visas and legal permanent residents are not affected, the Department of Homeland Security says dual nationals who travel on their passport from the unaffected country will also be allowed entry.
What about refugees?
The Supreme Court also approved a 120-day ban on refugees entering the US, allowing the government to bar entry to refugee claimants who cannot prove the same ties to an American individual or entity.
But opponents of the ban point out that refugees are invited to relocate in the US by the UN Refugee Agency, which has well-documented relationships with the people they refer for resettlement.
It remains unclear how many refugees scheduled to resettle in the US will be affected, but the government will fully halt the programme once its quota of 50,000 refugees for the budget year ending in September is met.
How did we get here?
The US president insisted his ban was necessary for national security and pointed to terrorist attacks in Paris, London, Brussels and Berlin as evidence.
However, critics called the policy un-American and Islamophobic, and said that this ban would not have stopped atrocities in the US perpetrated by American-born attackers.
The original ban, released on 27 January, provoked mass protests at American airports.
It included Iraq among nations whose travellers would be barred from the US, and imposed a full ban on refugees from Syria.
The president issued a revised version with a narrower scope on 6 March to overcome some of the legal problems.
The policy was left in limbo after it was struck down by federal judges in Hawaii and Maryland.